Development and clinical application of a strategy for PGD of single gene disorders combined with HLA matching
نویسندگان
چکیده
F.Fiorentino,,, A.Biricik, H.Karadayi, H.Berkil, G.Karlikaya, S.Sertyel, D.Podini, M.Baldi, M.C.Magli, L.Gianaroli and S.Kahraman EmbryoGen (Centre for Preimplantation Genetic Diagnosis), Rome, Reproductive Endocrinology and Genetics Unit, Istanbul Memorial Hospital, Istanbul, Turkey and GENOMA (Molecular Genetics Laboratory), Rome, Italy To whom correspondence should be addressed at: EmbryoGen (Preimplantation Genetic Diagnosis Centre), Via Po, 102 00198 Rome. Italy. E-mail: ®[email protected]
منابع مشابه
Novel One-Step Multiplex PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of β-Thalassemia
Preimplantation genetic diagnosis (PGD) of single gene disorders, combined with HLA matching (PGD-HLA), has emerged as a tool for couples at risk of transmitting a genetic disease to select unaffected embryos of an HLA tissue type compatible with that of an existing affected child. Here, we present a novel one-step multiplex PCR to genotype a spectrum of STRs to simultaneously perform HLA typin...
متن کاملThe Predictive Value of HLA-DR Matching and Cytokine Gene Polymorphisms in Renal Allograft Acute Rejection: A Living-unrelated Donor (LURD) Study
Background: In addition to Human Leukocyte Antigens (HLA) compatibility, gene polymorphisms in cytokines might also be important in the quality of allogeneic immune response. Objective: To evaluate the influence of HLA-DR matching and a number of cytokine gene polymorphisms on acute rejection after living-unrelated donor (LURD) kidney transplantation. Methods: A total of 42 renal transplants pe...
متن کاملO-27: Preimplantation Genetic Diagnosis in Prevention of Genetic Diseases -Diagnostic of Spinal Muscular Atrophy (SMA)
Background: Preimplantation genetic diagnosis - PGD is currently an established procedure allowing genetic research of oocyte or embryo before implantation to the uterus. Spinal muscular atrophy (SMA) is a neurodegenerative disorder, being the second most common lethal autosomal recessive disease in Caucasians, after cystic fibrosis. There are three clinically different types of which type I (W...
متن کاملPreimplantation diagnosis for Fanconi anemia combined with HLA matching.
CONTEXT The advent of single-cell polymerase chain reaction (PCR) has presented the opportunity for combined preimplantation genetic diagnosis (PGD) and HLA antigen testing. This is a novel and useful way to preselect a potential donor for an affected sibling requiring stem cell transplantation. OBJECTIVE To perform in vitro fertilization (IVF) and preimplantation HLA matching combined with P...
متن کاملI-17: The Ethics of Using IVF and PGD to Conceive A Savior Child
Until the advent of preimplantation genetic diagnosis (PGD) in the 90’s of the last century, the only efficient means to find out whether an offspring has inherited a genetic disease from a couple who are carriers of the defective gene is through prenatal diagnosis (PND). However, if the fetus is found to have the genetic disease, presently the only available treatment is to abort the fetus, an...
متن کامل